BLI Requirements
- Multiple analyte concentrations ranging from
- Injection time period long enough to see a curvature of the binding process
- association phase should have enough time - influencing
- should be enough dissociation time to see decay - influencing
- Time taken in each phase is a potential assumption?
- Its approximated - fast binding and dissociation, for example, would need less time
EQUILIBRIUM METHOD - Graph the different analyte concentrations against the R-eq values
- two variable fit results in Rmax and Kd
- Analogous to Michealis Menten formula