Single cycle kinetics
- in cases where the immobilized ligand can be inactivated irreversibly, several concentrations can be applied on a single cycle
- in contrast, multi-cycle assays required the regeneration of the immobilized ligand to test multiple analyte concentrations
These so called ‘‘kinetic titration series’’ or ‘‘single cycle kinetics’’ were found to be virtually as precise as classical ‘‘multi cycle kinetics’’ [4] and can be used as an alternative option if regeneration is not practical [5,6].
Kinetic Titration Series - Single Cycle Kinetics with BLI, page 1
- Karlsson R, Katsamba PS, Nordin H, Pol E, Myszka DG (2006) Analyzing a kinetic titration series using affinity biosensors. Analytical biochemistry 349: 136–147.
- This is a potential area of investigation