TitrationAnalysis - a tool for high throughput binding kinetics data analysis

Titration Analysis Tool Paper.pdf

• selected due to mention of error-propagation

In respect to how the paper handled errors associated with BLI

• Standard errors associated with $R_{max}$, $k_a$, $k_d$ are calculated through the Mathematica software used by the authors

  • Standard errors = statistical measure of how much uncertainty is associated with an estimated parameter
  • Standard errors are used to define precision confidence intervals

• Error propagation for $K_D$ was performed using the standard errors of $k_a$ and $k_d$

• The paper does not incorporate accuracy confidence intervals at all.