Important Notes

This page provides an overview of potential questions to answer, ordered from earliest to oldest:

Factors that impact the systematic error of k_a and k_d

Kinetic Titration Series - Single Cycle Kinetics with BLI

• question : Are there any differences in the errors associated to multi-cycle or single-cycle kinetics?

Octet Biomolecular binding kinetics

• practical method to solve for $k_a$ and $k_d$ - uses $k_{obs}$
• question : What’s better: $k_{obs}$ vs fitting for both $k_a$ and $k_d$

BioRad BLI Overview

• BLI Requirements: What influences $k_a$ and $k_d$ = practically assumptions
• question : How is $R_{eq}$ determined, it is unlikely that the association phase integrated rate equation would involve $K_D$ and $R_{max}$ or even by extension, $k_d/k_a$ as the Fundamentals of SPR Video